Deliverables
Deliverable 1.5 compiles the collection of 20 Practice Abstracts produced during the second period of the VIRTIGATION project, covering the new results, methodologies and knowledge generated between mid-2023 and the end of 2025.
Deliverable 5.6 describes the performance of plant genotypes claimed to be resistant or tolerant under field conditions to the emergent begomovirus Tomato leafcurl New Delhi virus (ToLCNDV) and the tobamovirus Tomato brown rugose fruit virus (ToBRFV).
Deliverable 4.5 addresses the emerging risks of multiple virus infections, by examining how new virus combinations affect particularly tomato and cucurbit crops, with a focus on resistance durability and commonly used management practices.
Deliverable 4.4 investigated the role of both viral and host factors contributing to the rapid and aggressive spread of ToLCNDV.
Deliverable 3.3 screened a selection of tomato and melon lines at pre-breeding stage for their resistance to ToLCNDV.
Deliverable 3.4 identified various molecular markers for whitefly resistance, and discusses a potential candidate gene present in the 13kb genomic region.
Deliverable D7.7 includes a summary of P3 consortium meetings minutes (final project meeting) held on 6th November 2025, and the final project conference held on 7th of November 2025 in Leuven, Belgium.
Deliverable D2.5 presents an integrated analysis of the diversity, evolution, and epidemiological drivers of two major plant viruses of global concern: Tomato leaf curl New Delhi virus (ToLCNDV) and Tomato brown rugose fruit virus (ToBRFV).
Deliverable D2.6 reports on the impact of Integrated Pest Management (IPM) strategies on the genetic diversity of key plant viruses affecting tomatoes and cucurbits within the VIRTIGATION project.
Deliverable D3.5 provides a map of key genes involved in plant response to viral infections, in particular to ToBRFV infections.
Deliverable D5.7 researched the feasibility of several integrated management strategies for two key viral pathosystems: the mechanically transmitted tomato brown rugose fruit virus (ToBRFV), and the whitefly-transmitted tomato leaf curl New Delhi virus (ToLCNDV).
Deliverable D6.7 provides a final report on the dissemination & communication (D&C) tools and activities of the project, focusing in particular on project period 3 (P3), which lasted from M37-M54.
Deliverable D6.8 provides a comprehensive mapping of exploitable results, with clear indications of ownership, protection measures, and intended exploitation routes.
Deliverable D7.6 consolidates the approach to ensuring responsible, transparent, and sustainable management of research data, in accordance with Article 29.3 of the Horizon 2020 Grant Agreement, and VIRTIGATION’s voluntary participation in the Open Research Data Pilot (ORDP) of the Horizon 2020 funding programme.
Deliverable D5.4 provides a technical report on the possibility of using a cross-protection approach to control ToBRFV in greenhouse tomato crops.
Deliverable D4.1 provides a technical report on the influence of climate change on virus vector interaction for ToLCNDV as its vector, the Bemisia tabaci whitefly.
Deliverable D5.3 identifies methods to eradicate tobamoviruses in contaminated growing media. Specifically, the application of heat was investigated. Two methods were tested: steaming and solarisation.
Deliverable D6.6 includes a detailed planning for the third and final project period of VIRTIGATION’s DEC strategy, as well as all planned DEC tools, materials and activities.
Deliverable D6.5 includes a summary of the dissemination & communication tools, materials and activities produced in the second project period of VIRTIGATION.
Deliverable D7.5 includes a summary of all consortium meetings minutes carried out during the second
project period of VIRTIGATION.
Deliverable 5.5 describes the experimental tests realised in semi-field and in field environment to screen a selection of
plant extracts with insecticidal effects, developed in the frame of the project, for: i) their efficacy on whitefly vectors, especially Bemisia spp.gr. tabaci; ii) their selectivity on natural enemies (both parasitoids and predators), and iii) their side effects on pollinator insects.
Deliverable 5.2 is exploring the possibility to develop a cross-protection strategy against ToBRFV for control of the virus in tomato crop. In order to optimize the chance of finding a suitable cross-protection candidate isolate, different research strategies were applied, including screening of naturally occurring ToBRFV isolates, as well as creation of attenuated isolates.
Deliverable 4.3 investigated the competence of different whitefly species transmitting ToLCNDV, as well as the alternative host plants for ToLCNDV and ToBRFV.
Deliverable 4.2 investigated the possibilities for the long-distance dispersal of viruses via seed. We focused our work on the two fast spreading viruses in Europe: the tomato leaf curl New Delhi virus (ToLCNDV) and tomato brown rugose fruit virus (ToBRFV). We investigated both ‘seed-borne’ and ‘seed-transmitted’ nature of both viruses.
Deliverable 3.2 developed near-isogenic tomato lines (NIL) carrying all possible combinations of the four known TYLCV-resistant loci, Ty-2 through ty-5, in the same genetic background. The NIL’s
were inoculated with either TYLCV alone, or with TYLCV and betasatellite, and tested for their
resistance level. Indeed, it was found that tomato lines expressing a combination of TYLCV-resistance genes exhibited a very high level of resistance to both TYLCV and betasatellite.
Deliverable 2.4 outlines the changes made to the Genome Detective Platform to accommodate the collection and analysis of plant virus samples, including metagenomic viral sequences and epidemiological metadata annotations, for the analysis of plant virus outbreaks.
Deliverable 2.3 compiles the protocols for full length genome sequencing of circular DNA and linear RNA viruses designed to study virus populations, their diversity, geographical distribution and evolution.
Deliverable 1.3 presents a comprehensive report of the stakeholder network of the VIRTIGATION project gathered so far in regards of the multi-actor approach, and further includes proposed actions and guidelines to enhance the stakeholders participation. It builds on the initial internal guidelines produced at the onset of the project (cf. D1.1).
Deliverable 1.4 compiles 14 practice abstracts of the results and knowledge generated during the first period (M1-M18) of the VIRTIGATION project. It also presents the results of co-creation activities performed with the help of National Knowledge Brokers in the project’s focus countries.
Deliverable 7.4 provides a major update of the initial DMP (cf. D7.2) at the mid-term of VIRTIGATION’s duration, and now also includes a summarised description of the project’s generated, as well as collected and processed research datasets.
Deliverable 6.4 provides a first report on the actual exploitable results attained up till now at mid-term of the VIRTIGATION project, and further outlines the measures taken so far by result owners to ensure the (post-)project use of their results.
Deliverable 6.3 provides an update at mid-way in project implementation on the initial DEC plan (cf D6.1).
Deliverable 7.3 provides a summary of all consortium meeting minutes and the established activity plans for the following period P2.
Deliverable 6.2 provides a report summarising analyses of all thus far implemented communication and dissemination materials, tools and channels.
Deliverable 3.1 aims at finding ToBRFV resistance in tomato wild relatives and to introgress them into tomato cultivars.
Deliverable 5.1 aims at finding plant extracts with insecticidal effect against the whitefly vector (Bemisia tabaci, MED-type). For this purpose, 12 different candidate plant extracts were tested against various developmental stages of whitefly under controlled laboratory conditions. All experiments were conducted on tomato host (cv. Moneymaker).
The sample collection deliverable D2.2 includes a list of virus infected plant samples collected in participating countries that will be used to develop protocols for full length viral genome sequencing, study virus populations, their diversity, geographical distribution and evolution.
The list of identified actors in the VIRTIGATION network deliverable D1.2 includes a categorized list of stakeholders in the project’s multi-actor network, compiled by National Knowledge Brokers and national partners.
The sample descriptor list deliverable D2.1 includes the list of biological descriptors that will be used to determine the origin, negative impact and dissemination of plant viruses.
The initial data management plan deliverable D7.2 describes the project’s data management strategy in compliance with its participation in the EU Horizon 2020 Open Research Data Pilot. It also provides naming and metadata guidelines.
The project manual deliverable D7.1 defines the VIRTIGATION consortium operating procedures and summarizes all the required knowledge and best practices for the effective management of the project.
The initial internal guidelines for multi-actor approach and co-creation deliverable D1.1 guides the VIRTIGATION consortium through the multi-actor approach and co-creation process implemented in the project. It also identifies the most relevant actors and stakeholders for the VIRTIGATION network.
The initial DEC plan and DEC handbook deliverable D6.1 provides guidelines on dissemination, exploitation and communication strategies, tools and channels. It also details what is required from VIRTIGATION partners for effective exploitation and how to ensure open access to peer-reviewed scientific publications.
The Ethics H-Requirement deliverable D8.1 outlines how the VIRTIGATION project will fulfil procedures and criteria for the informed participation of research subjects.
The Ethics POPD-Requirement deliverable D8.2 describes how the processing of personal data of research subjects will be ensured in compliance with the General Data Protection Regulation (GDPR).
The Ethics NEC-Requirement deliverable D8.3 details how the project will ensure fair benefit-sharing with stakeholders from low- and lower-middle income countries.
The Ethics EPQ-Requirement deliverable D8.4 provides information on how the conducted research and measures will be compliant with appropriate environment, health and safety procedures.
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